Enzymatic Histochemistry of Choline-Deficient Cirrhosis

Studies on Enzymatic Histochemistry

Extraction experiments demonstrated that choline esterase could be removed from microtome sections of tissue with as great facility by 0.9 per cent NaCl as by 30 per cent glycerol. The quantitative distribution of choline esterase through the wall of the pig stomach was studied, and it was found that the epithelial cell region possessed the greatest activity and muscle tissue the least. Pylorus...

STUDIES ON ENZYMATIC HISTOCHEMISTRY XXVI. Tm~ HISTOLOGICAL DISTRIBUTION O1~ CHOLINE ESTERASE IN THE GASTRIC MUCOSA NORMALLY

In previous papers of this series Linderstr¢fm-Lang, Holter, and coworkers have investigated the histological distribution of pepsin (1, 2), acid (2, 3), peptidase (2, 4), and esterase (2, 5) in the gastric mucosa of pigs, and the urease (6) in dog's stomach. The present investigation is a continuation of this work with regard to choline esterase. In view of the well known theory of chemical me...

Choline Dihydrogen Citrate in Infantile Biliary Cirrhosis

Steroid catabolism in choline-deficient rats.

Dolly, J. O., Dillon, A., Duffy, M. J. & Fottrell, P. F. (1971) Clin. Chim. Actu 31, 55-62 Donlon, J. & Fottrell, P. F. (1971) Clin. Chim. Acta 33, 345-350 Donlon, J. & Fottrell, P. F. (1972) Cornp. Biochem. Physiol. B 41, 181-193 Fogel, M. R. & Adibi, S. A. (1974) J. Lab. Clin. Med. 84, 327-333 OCuinn, G. & Fottrell, P. F. (1975) Biochem. Soc. Trans. 3, 1219-1221 OCuinn, G., Piggott, C. 0. & F...

Enzymatic measurement of choline-containing phospholipids in bile.

An enzymatic method, previously used to determine choline-containing phospholipids in serum, was applied to the estimation of phospholipid concentration in bile. The method combines three enzymatic reactions: a) release of choline by phospholipase D; b) choline oxidation by choline oxidase, a reaction which generates hydrogen peroxide; and c) formation of a red quinone dye by peroxidase. The me...